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trap staining kit 387a  (Merck KGaA)

 
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    Merck KGaA trap staining kit 387a
    gCTRP3 inhibits bone resorption and promotes bone formation. (A) Pathological changes of the left femurs of OVX-induced mice were observed using hematoxylin and <t>eosin</t> <t>staining</t> following treatment with gCTRP3 (scale bar, 25 µm). (B) Number of osteoclasts in the left femurs of OVX-induced mice were observed using <t>TRAP</t> staining following treatment with gCTRP3 (scale bar, 50 µm). (C) RUNX2 expression levels in the left femurs of OVX-induced mice were detected using immunofluorescence analysis following treatment with gCTRP3 (scale bar, 50 µm). (D) CTX-1, TRAP, OCN and P1NP expression levels in the serum of OVX-induced mice were detected using ELISA kits following treatment with gCTRP3. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. OVX group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; OVX, oophorectomy; E2, estradiol valerate; TRAP, tartrate-resistant acid phosphatase; RUNX2, Runt-related transcription factor-2; CTX-1, C-telopeptide of type I collagen; OCN, osteocalcin; P1NP, procollagen type 1 N-terminal propeptide.
    Trap Staining Kit 387a, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/387a+kit/trap+staining+kit/pmc11157184-53-0-8
    Average 90 stars, based on 1 article reviews
    trap staining kit 387a - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "gCTRP3 inhibits oophorectomy‑induced osteoporosis by activating the AMPK/SIRT1/Nrf2 signaling pathway in mice"

    Article Title: gCTRP3 inhibits oophorectomy‑induced osteoporosis by activating the AMPK/SIRT1/Nrf2 signaling pathway in mice

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2024.13257

    gCTRP3 inhibits bone resorption and promotes bone formation. (A) Pathological changes of the left femurs of OVX-induced mice were observed using hematoxylin and eosin staining following treatment with gCTRP3 (scale bar, 25 µm). (B) Number of osteoclasts in the left femurs of OVX-induced mice were observed using TRAP staining following treatment with gCTRP3 (scale bar, 50 µm). (C) RUNX2 expression levels in the left femurs of OVX-induced mice were detected using immunofluorescence analysis following treatment with gCTRP3 (scale bar, 50 µm). (D) CTX-1, TRAP, OCN and P1NP expression levels in the serum of OVX-induced mice were detected using ELISA kits following treatment with gCTRP3. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. OVX group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; OVX, oophorectomy; E2, estradiol valerate; TRAP, tartrate-resistant acid phosphatase; RUNX2, Runt-related transcription factor-2; CTX-1, C-telopeptide of type I collagen; OCN, osteocalcin; P1NP, procollagen type 1 N-terminal propeptide.
    Figure Legend Snippet: gCTRP3 inhibits bone resorption and promotes bone formation. (A) Pathological changes of the left femurs of OVX-induced mice were observed using hematoxylin and eosin staining following treatment with gCTRP3 (scale bar, 25 µm). (B) Number of osteoclasts in the left femurs of OVX-induced mice were observed using TRAP staining following treatment with gCTRP3 (scale bar, 50 µm). (C) RUNX2 expression levels in the left femurs of OVX-induced mice were detected using immunofluorescence analysis following treatment with gCTRP3 (scale bar, 50 µm). (D) CTX-1, TRAP, OCN and P1NP expression levels in the serum of OVX-induced mice were detected using ELISA kits following treatment with gCTRP3. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. OVX group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; OVX, oophorectomy; E2, estradiol valerate; TRAP, tartrate-resistant acid phosphatase; RUNX2, Runt-related transcription factor-2; CTX-1, C-telopeptide of type I collagen; OCN, osteocalcin; P1NP, procollagen type 1 N-terminal propeptide.

    Techniques Used: Staining, Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Control

    Related Articles

    Staining:

    Article Title: 4-Hexylresorcinol inhibits osteoclastogenesis by suppressing the NF-κB signaling pathway and reverses bone loss in ovariectomized mice
    Article Snippet: .. TRAP staining was performed using a 387A kit (Merck KGaA), Briefly, a mixed solution of citrate solution, Fast Gamet GBC Base solution, naphthol AS-BI phosphonic acid solution and sodium nitrite solution were preheated at 37 ̊C before tartrate solution was added to the mixture, the slides were then submerged into the mixed solution for 1 h at 37 ̊C as previously described ( ). ..

    Article Title: gCTRP3 inhibits oophorectomy‑induced osteoporosis by activating the AMPK/SIRT1/Nrf2 signaling pathway in mice
    Article Snippet: .. TRAP staining was performed using a 387A kit (Merck KGaA), Briefly, a mixed solution of citrate solution, Fast Gamet GBC Base solution, naphthol AS-BI phosphonic acid solution and sodium nitrite solution were preheated at 37°C before tartrate solution was added to the mixture, the sections were then submerged into the mixed solution for 1 h at 37°C. .. Stained sections were photomicrographed using a light microscope (Olympus Corp.).



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    gCTRP3 inhibits bone resorption and promotes bone formation. (A) Pathological changes of the left femurs of OVX-induced mice were observed using hematoxylin and <t>eosin</t> <t>staining</t> following treatment with gCTRP3 (scale bar, 25 µm). (B) Number of osteoclasts in the left femurs of OVX-induced mice were observed using <t>TRAP</t> staining following treatment with gCTRP3 (scale bar, 50 µm). (C) RUNX2 expression levels in the left femurs of OVX-induced mice were detected using immunofluorescence analysis following treatment with gCTRP3 (scale bar, 50 µm). (D) CTX-1, TRAP, OCN and P1NP expression levels in the serum of OVX-induced mice were detected using ELISA kits following treatment with gCTRP3. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. OVX group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; OVX, oophorectomy; E2, estradiol valerate; TRAP, tartrate-resistant acid phosphatase; RUNX2, Runt-related transcription factor-2; CTX-1, C-telopeptide of type I collagen; OCN, osteocalcin; P1NP, procollagen type 1 N-terminal propeptide.
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    Image Search Results


    gCTRP3 inhibits bone resorption and promotes bone formation. (A) Pathological changes of the left femurs of OVX-induced mice were observed using hematoxylin and eosin staining following treatment with gCTRP3 (scale bar, 25 µm). (B) Number of osteoclasts in the left femurs of OVX-induced mice were observed using TRAP staining following treatment with gCTRP3 (scale bar, 50 µm). (C) RUNX2 expression levels in the left femurs of OVX-induced mice were detected using immunofluorescence analysis following treatment with gCTRP3 (scale bar, 50 µm). (D) CTX-1, TRAP, OCN and P1NP expression levels in the serum of OVX-induced mice were detected using ELISA kits following treatment with gCTRP3. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. OVX group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; OVX, oophorectomy; E2, estradiol valerate; TRAP, tartrate-resistant acid phosphatase; RUNX2, Runt-related transcription factor-2; CTX-1, C-telopeptide of type I collagen; OCN, osteocalcin; P1NP, procollagen type 1 N-terminal propeptide.

    Journal: Molecular Medicine Reports

    Article Title: gCTRP3 inhibits oophorectomy‑induced osteoporosis by activating the AMPK/SIRT1/Nrf2 signaling pathway in mice

    doi: 10.3892/mmr.2024.13257

    Figure Lengend Snippet: gCTRP3 inhibits bone resorption and promotes bone formation. (A) Pathological changes of the left femurs of OVX-induced mice were observed using hematoxylin and eosin staining following treatment with gCTRP3 (scale bar, 25 µm). (B) Number of osteoclasts in the left femurs of OVX-induced mice were observed using TRAP staining following treatment with gCTRP3 (scale bar, 50 µm). (C) RUNX2 expression levels in the left femurs of OVX-induced mice were detected using immunofluorescence analysis following treatment with gCTRP3 (scale bar, 50 µm). (D) CTX-1, TRAP, OCN and P1NP expression levels in the serum of OVX-induced mice were detected using ELISA kits following treatment with gCTRP3. ***P<0.001 vs. Control group; # P<0.05, ## P<0.01 and ### P<0.001 vs. OVX group. gCTRP3, globular C1q/tumor necrosis factor-related protein 3; OVX, oophorectomy; E2, estradiol valerate; TRAP, tartrate-resistant acid phosphatase; RUNX2, Runt-related transcription factor-2; CTX-1, C-telopeptide of type I collagen; OCN, osteocalcin; P1NP, procollagen type 1 N-terminal propeptide.

    Article Snippet: TRAP staining was performed using a 387A kit (Merck KGaA), Briefly, a mixed solution of citrate solution, Fast Gamet GBC Base solution, naphthol AS-BI phosphonic acid solution and sodium nitrite solution were preheated at 37°C before tartrate solution was added to the mixture, the sections were then submerged into the mixed solution for 1 h at 37°C.

    Techniques: Staining, Expressing, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Control